Hi Mike and David, first of all: thanks for your effort!
I have used the same database for both searches. If I look on the xml result file of the 1.6.1 search, I see reverse hits. The database is fine, all reverse hits start with REVERSE_ I did not enable accurate mass binning because I just wanted to try the default setting first, but if it's better for high res. data then I will switch it on in the future. Thank you! Thomas Am Donnerstag, 19. September 2019 19:37:23 UTC+2 schrieb David Shteynberg: > > Hi Mike and Thomas, > > I am not sure where Kojak search went wrong, but somehow the 1.6.1 search > contains 0 decoys ("REVERSE") tagged hits and the 1.5.5 search has many. > Can you check your database and search parameters? Also is there any > reason your are not enabling the ACCMASS option that will help the > classification? > > Thanks, > -David > > On Thu, Sep 19, 2019 at 4:48 AM 'Michael Hoopmann' via spctools-discuss < > spctools...@googlegroups.com <javascript:>> wrote: > >> Hi Thomas, >> Sorry for the slow response, but HUPO is over now. I'll help take a look >> and find the problem. >> Cheers, >> Mike >> >> On Sun, Sep 15, 2019 at 11:56 PM Thomas Gossenreiter < >> thomas.go...@gmail.com <javascript:>> wrote: >> >>> Please find the links to the files below: >>> >>> https://drive.google.com/open?id=1bO2E0duY8gr3UCoMRClKhKlEBiLqG88D >>> https://drive.google.com/open?id=15BKZkfBfPsMROSvrxBOK9XtCivHG7nHk >>> >>> >>> Am Freitag, 13. September 2019 16:27:47 UTC+2 schrieb David Shteynberg: >>>> >>>> It would be very helpful to see your Kojak pepxml results. >>>> >>>> Thanks, >>>> David >>>> >>>> >>>> On Fri, Sep 13, 2019, 12:01 AM Thomas Gossenreiter < >>>> thomas.go...@gmail.com> wrote: >>>> >>>>> Hi David, >>>>> >>>>> thanks for your quick response. Here is the link to the zipped pep.xml >>>>> files. Is this enough or do you also need the pep.xmls from the kojak >>>>> search? >>>>> >>>>> https://drive.google.com/open?id=1zKW0JVfVL_LME0gmt_L6AI7j8AymUmD1 >>>>> >>>>> >>>>> Am Donnerstag, 12. September 2019 18:02:07 UTC+2 schrieb David >>>>> Shteynberg: >>>>>> >>>>>> Hi Thomas, >>>>>> >>>>>> I am not sure what is happening but if you could provide the dataset >>>>>> files for the two search results I can investigate. PeptideProphet >>>>>> does >>>>>> calculate different models for the different crosslink types. >>>>>> >>>>>> Thanks, >>>>>> -David >>>>>> >>>>>> On Thu, Sep 12, 2019 at 8:59 AM Thomas Gossenreiter < >>>>>> thomas.go...@gmail.com> wrote: >>>>>> >>>>>>> Hi, >>>>>>> >>>>>>> I have used Kojak 1.6.1 in the TPP pipeline and got some strange >>>>>>> results (very similar ms2 spectra from one peptide with similar Kojak >>>>>>> scores got very different probabilities when running peptide prophet). >>>>>>> I >>>>>>> have plotted the Kojak score vs. the peptide prophet probability for >>>>>>> target >>>>>>> and decoy hits and there is hardly any correlation between the kojak >>>>>>> score >>>>>>> and the probability. >>>>>>> >>>>>>> I have repeated the search Kojak version that comes along with TPP >>>>>>> (1.5.5) using the same settings except for the top_count parameter. I >>>>>>> think >>>>>>> this looks how it should, except for the cross-links which are scored >>>>>>> very >>>>>>> well by Kojak but get a bad peptide prophet probability. Is this the >>>>>>> case >>>>>>> when one peptide has a very good Kojak sub score and the other one a >>>>>>> very >>>>>>> bad score? >>>>>>> >>>>>>> Does version 1.6.1 work for you or do you have any idea what is >>>>>>> happening here? I attached a ppt file with the figures. >>>>>>> >>>>>>> I also got a general questions: Is peptide prophet calculating FDRs >>>>>>> separately for cross-linked, loop-linked and normal peptides? >>>>>>> >>>>>>> Kind regards, >>>>>>> Thomas >>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> -- >>>>>>> You received this message because you are subscribed to the Google >>>>>>> Groups "spctools-discuss" group. >>>>>>> To unsubscribe from this group and stop receiving emails from it, >>>>>>> send an email to spctools...@googlegroups.com. >>>>>>> To view this discussion on the web visit >>>>>>> https://groups.google.com/d/msgid/spctools-discuss/12f12195-f115-41e5-a196-b53e18e170df%40googlegroups.com >>>>>>> >>>>>>> <https://groups.google.com/d/msgid/spctools-discuss/12f12195-f115-41e5-a196-b53e18e170df%40googlegroups.com?utm_medium=email&utm_source=footer> >>>>>>> . >>>>>>> >>>>>> -- >>>>> You received this message because you are subscribed to the Google >>>>> Groups "spctools-discuss" group. >>>>> To unsubscribe from this group and stop receiving emails from it, send >>>>> an email to spctools...@googlegroups.com. >>>>> To view this discussion on the web visit >>>>> https://groups.google.com/d/msgid/spctools-discuss/abe43fc7-eb03-4411-a6ac-c5ca4a5c326a%40googlegroups.com >>>>> >>>>> <https://groups.google.com/d/msgid/spctools-discuss/abe43fc7-eb03-4411-a6ac-c5ca4a5c326a%40googlegroups.com?utm_medium=email&utm_source=footer> >>>>> . >>>>> >>>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to spctools...@googlegroups.com <javascript:>. >>> To view this discussion on the web visit >>> https://groups.google.com/d/msgid/spctools-discuss/71e9da68-1b77-4913-b2f0-049275e04709%40googlegroups.com >>> >>> <https://groups.google.com/d/msgid/spctools-discuss/71e9da68-1b77-4913-b2f0-049275e04709%40googlegroups.com?utm_medium=email&utm_source=footer> >>> . >>> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools...@googlegroups.com <javascript:>. >> To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/CANMWFMNVu2gfVxcUq_Pbttrx7WZOJHCsOtk6VOZb-H6THUtLTA%40mail.gmail.com >> >> <https://groups.google.com/d/msgid/spctools-discuss/CANMWFMNVu2gfVxcUq_Pbttrx7WZOJHCsOtk6VOZb-H6THUtLTA%40mail.gmail.com?utm_medium=email&utm_source=footer> >> . >> > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. 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