Dear all,
I am trying to model a weak dimer using intermolecular PCS. I have intermolecular PCS from natural abundance protein bound to lanthanides mixed with [U-15N] labeled protein that’s diamagnetic. Therefore, the PCS that we are observing only come from the dimer. Since the complex is weak, the PCS that I am measuring are scaled by the small fractional population of the dimer and are smaller than they would be for a 100% bound complex. I am wondering if anyone has any experience with the appropriate way to deal with the measured PCS to account for the small population of dimer? I have an estimate of the Kd that I can use to calculate the fractional population of dimer but I want to make sure I use the appropriate treatment of the PCS. Thank you in advance for any help. Best regards, Taylor -- Taylor R. Cole Graduate Student Texas A&M University [email protected]
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