Hello Taylor-- > > I am trying to model a weak dimer using intermolecular PCS. I have > intermolecular PCS from natural abundance protein bound to lanthanides mixed > with > [U-15N] labeled protein that’s diamagnetic. Therefore, the PCS that we are > observing only come from the dimer. > > Since the complex is weak, the PCS that I am measuring are scaled by the small > fractional population of the dimer and are smaller than they would be for a > 100% > bound complex. > > > > I am wondering if anyone has any experience with the appropriate way to deal > with > the measured PCS to account for the small population of dimer? > > I have an estimate of the Kd that I can use to calculate the fractional > population of dimer but I want to make sure I use the appropriate treatment of > the PCS. >
If only a single species contributes to the PCS, I don't see any complicating factor, as long as you see a signal. Of course, things are complicated if there are multiple contributions. best regards-- Charles _______________________________________________ Xplor-nih mailing list [email protected] https://dcb.cit.nih.gov/mailman/listinfo/xplor-nih
