OK. Thanks, Donna.

Zhiwei






At 2014-10-30 22:29:32, "Donna Dierker" <do...@brainvis.wustl.edu> wrote:
>I'm sorry I don't know the answer to the Freesurfer question, but someone else 
>might know.  And I confess I don't understand the rationale for thresholding 
>at that value, possibly because I am unfamiliiar with the contents of those 
>files.
>
>So I'm keeping the responsibility for ensuring a reasonable threshold on you. 
>;-)  But If it's each subject, then it's probably worth scripting it using 
>these caret_command tools:
>
>      caret_command -surface-region-of-interest-selection  
>         [-metric  metric-file-name  column  min  max SEL-TYPE]
>
>      caret_command -surface-roi-statistical-report  
>
>And you can use each subject's surface/topo for that surface area calculation. 
> Your min is your threshold and max something like 9999999.  Your report will 
>include the area of the suprathreshold regions, and you can grep that line 
>from the resulting report file.
>
>
>On Oct 29, 2014, at 11:30 PM, wangzhiwei3233 <wangzhiwei3...@126.com> wrote:
>
>> Hi, Donna,
>> 
>> My purpose is counting activated area size on each subject.
>> 
>> I did do significance test using Freesurfer on individual level. The results 
>> contained many files,for example, "sig.nii.gz" and "Fsig.nii.gz" 
>> corresponding to result of t and f test respectively. Is that right?
>> But I do not know how to determine tha suprathreshold on subject level as 
>> you mentioned. 
>> 
>> For display and counting area size, I converted the results file(sig.nii.gz) 
>> to Caret. Then I count area size on Caret. When I counted area size, I 
>> selected a uniform threshold 1.3, i.e.-log10(0.05) for each subject. So I 
>> set the scale to 1.3 ~ maxminum on Caret. Is this right?
>> 
>> So I could draw a border around the  suprathreshold region and generated a 
>> paint file. I  got the area size of the region using the paint file.
>> 
>> Is there any step wrong?
>> 
>> Thanks!
>> Zhiwei
>> 
>> 
>> 
>> At 2014-10-30 00:27:14, "Donna Dierker" <do...@brainvis.wustl.edu> wrote:
>> >Hi wangzhiwei,
>> >
>> >I'm a little confused by the question.  There mention of area size and 
>> >scales hints that there might be a confusion between tools used for 
>> >quantification / significance testing and those used for display purposes.
>> >
>> >Freesurfer has its own tools for significance testing, so you could use 
>> >those.  We often use threshold-free cluster enhancement for that purpose, 
>> >which finds the significance threshold.  Suprathreshold area can be 
>> >computed once this threshold has been determined.
>> >
>> >But usually when I make a figure, I generate border around the 
>> >suprathreshold regions and display these bordersover the "real" t- or 
>> >f-map, using a scale that corresponds to my alpha (e.g., .05) divided by 
>> >two (since I usually do both right and left hem tests).  I compute this t 
>> >or f-stat using my n / degrees of freedom.
>> >
>> >So the significance testing and display steps are separate, the way I do it.
>> >
>> >Now you might not be going as far as significance testing.  Sometimes you 
>> >just want to look at some preliminary data -- particularly for a single 
>> >subject.  A good start might be to understand if this is a single subject, 
>> >group results, what kind of statistic.
>> >
>> >And certainly not everyone does this the way I do, so it would be helpful 
>> >for others to weigh in with their viewpoints/conventions.
>> >
>> >Donna
>> >
>> >
>> >On Oct 28, 2014, at 9:53 PM, wangzhiwei3233 <wangzhiwei3...@126.com> wrote:
>> >
>> >> Hi, experts,
>> >> I converted fMRI results derived from freesurfer to caret, and not I want 
>> >> to count activation areas on caret. So there is a problem of threshold 
>> >> and scale.
>> >> 
>> >> Auto scale range is 0~60. I found that the area size was different when 
>> >> using scale 1.3~4 from when using scale 1.3~60. And the latter one was 
>> >> smaller. However , in the latter case(1.3~60),  the value of a point that 
>> >> was next to the border of activation area but in non-activation area was 
>> >> a little bit lager than the threshold 1.3. 
>> >> 
>> >> How to set the scale to guarantee the activation accurate?
>> >> 
>> >> Best!
>> >> 
>> >> 
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>> >
>> >
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