Dear all,
We had collected a complete MAD dataset for our 40 KDa protein. Mass-spect results shows incorporation of 10 seleno-methionines. But we could identify only 6 out of the 10 Seleno-methionine sites. The intial map (bnp) isn't really good. With that, we have completed the structure partially and at this stage we are a bit stuck. Is there a way we can nail down the minor sites as well, which can be of great help in fixing the remaining parts of the structure.
Thanks and Regards,
Dileep
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Dileep Vasudevan
Graduate Student
Dept of Biological Sciences
National University of Singapore
Singapore 117543
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