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Dear Dileep, feed these initial 6 sites into autoSHARP (together with the MAD MTZ file and your sequence). If there is any signal in the data strong enough to show you the additiona couple of sites, autoSHARP should complete it for you. Cheers Clemens On Tue, Oct 11, 2005 at 07:40:29PM +0800, Dileep Vasudevan wrote: > Dear all, > > We had collected a complete MAD dataset for our 40 KDa protein. > Mass-spect results shows incorporation of 10 seleno-methionines. But we > could identify only 6 out of the 10 Seleno-methionine sites. The intial > map (bnp) isn't really good. With that, we have completed the structure > partially and at this stage we are a bit stuck. Is there a way we can > nail down the minor sites as well, which can be of great help in fixing > the remaining parts of the structure. > > Thanks and Regards, > Dileep > > ------------------------------------------------ > Dileep Vasudevan > Graduate Student > Dept of Biological Sciences > National University of Singapore > Singapore 117543 > ----------------------------------------------- > > > -- *************************************************************** * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com * * Global Phasing Ltd. * Sheraton House, Castle Park * Cambridge CB3 0AX, UK *-------------------------------------------------------------- * BUSTER Development Group (http://www.globalphasing.com) ***************************************************************
