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Hello again... Abou the problem I´m having I´ll give more details so that you can help me... I really don´t think that the problem is the space group... The model I first used was the native lectin, and the correlation was 99,0 % while the Rfactor was 0,374. I carried out the rigid body refinement whic gave a 0,296 Rfactor and a 0,279 Rfree The restrained refinement made the Rfactor drop to0,16, but the Rfree increased to 30,2. I´ve fit the sugar in its density and created a pdb that already included the ligand in it, then I used it to do a new molecular replacement. The density of the ligand improved but further R free and Rfactor behaved very similarly to what has been descrided before. Thanks for the attention -- Taianá Maia de Oliveira Laboratório de Moléculas Biologicamente Ativas - Biomol-lab Departamento de Bioquímica e Biologia Molecular
