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Hello Jaishin, First of all, you should look at your MR-solution as soon as possible: Once you obtained the MR-solution, only run a rigid body refinement, not a 'normal' refinement. I believe that phaser already carries out a rigid body refinement with the solution, so you can look at the map straight away. The reason for this is that refinement tries to flatten your difference map, and after MR it is those differences that you do want to see in order to correct for the differences between the search model and your data. Secondly, you could split the search model into two parts and then search for the first one, then for the second with the first one fixed. This might place both parts correctly. Tim -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Mon, 1 May 2006, [big5] ¼B®aªY(OZ) wrote: > Dear ALL: > > A ambigous phase problem. My dataset was colleted and indexed as R3/H3 > space group and subsquently into processed molecular replacement method > with 30% sequence identity of the template.There were 2 indicated > solutions obtained from the MR. After refinement, R /free R value go > down to 0.33/0.37. > Inspection with the resulted file, 3D model reveals a crescent-shaped > structure formed 2 lobes. A deep cleft was embeedded between the lobes. > However, only the residues of N-terminal lobe was fitted with the > density map. The other one was not fitted with density map. All attempts > to automatic build mainchain was failure, even manual build was > difficult to work. About 13 degree conformational change of the lobes of > the template was described before in references. Any suggestons about > the phase improvement? Thanks a lot. > > > jaishin
