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Hi Zhen, Have a look at http://www.dl.ac.uk/list-archive-public/ccp4bb/msg17757.html (and links therein) for a lively discussion on these matters. HTH Peter [EMAIL PROTECTED] wrote:
*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Deal all, I recently solved a structure at 1.7A by MAD. However, there is always strong negative density arround all 8 selenium in the protein during CNS refinement. The Selenium-methionines fit 2fo-fc density well. I tried to use peak, edge or remote dataset with or without scale anomalous flag on and all did not matter. Does anybody have a solution for this matter? Thanks. Zhen
