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This is indirectly crystallization-related:
Can anyone comment on the usefulness of Poros MC resin for purification of
His-tagged proteins, especially in comparison to the various Amersham
products? I'm dealing with a protein that is happiest with 20% glycerol
in the initial buffer, and this makes any FPLC- or pump-based purification
very slow due to the relatively low pressure tolerance of Sepharose
media. The Poros ion exchange resin works very well and is both robust
and cheap, but the MC resin is about 5 times more expensive than anything
else I've found. Other than the price, is there any reason not to use
it?
(For what it's worth, batch purification with Sepharose works relatively
well for me, but I've found it too cumbersome in practice and I'm trying
to scale up with multiple constructs in this particular case.)
thanks,
Nat
