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The silica-based Ni-TED resin sold by Macherey-Nagel can take 10 MPa
pressure
(~1400 psi for the SI-challenged) while the Poros MC resin takes 17 MPa
(~2400 psi). Last time I checked, Ni-TED resin was sold at about the
same price as the
1 MPa Pharmacia Sepharose (Swedish prices). Make sure that all parts
of your purification
setup can take these pressures.
Cheers,
Martin
.
B. Martin Hallberg, PhD
Structural Genomics Consortium
Department of Medical Biochemistry and Biophysics
Karolinska Institutet
Scheeles väg 2
SE-171 77 Stockholm
http://sgc.ki.se/groupSB2.html
On Oct 8, 2006, at 8:10 AM, Nat Echols wrote:
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This is indirectly crystallization-related:
Can anyone comment on the usefulness of Poros MC resin for
purification of His-tagged proteins, especially in comparison to
the various Amersham products? I'm dealing with a protein that is
happiest with 20% glycerol in the initial buffer, and this makes
any FPLC- or pump-based purification very slow due to the
relatively low pressure tolerance of Sepharose media. The Poros
ion exchange resin works very well and is both robust and cheap,
but the MC resin is about 5 times more expensive than anything else
I've found. Other than the price, is there any reason not to use it?
(For what it's worth, batch purification with Sepharose works
relatively well for me, but I've found it too cumbersome in
practice and I'm trying to scale up with multiple constructs in
this particular case.)
thanks,
Nat
