Jenny
On 10/24/06, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote:
Hi, Jenny :
One week is generally considered as good time. From our experience, anything
grows within one day is usually low resolution and high mosaicity. You can try
to use microseeding to get bigger crystals. The way I do is to put some crystals
in 100ul mother liquer and crash them by votexing with a teflon bead and use
this solution to add to your protein solution instead of mother liquer.
I noticed that your crystallization condition is AS. What is your cryo
protectant? Did you try the wet mount to see if you have right cryo protectant?
For AS, I will recommend you to try add 15% or 30% glucose to your mother liqure
and put your crystal in this cryo protectant around 5 mins. Our lab has several
successful experience by using glucose as cyro protectant.
Good luck
Meng-Chiao Joseph Ho
> *** CCP4 home page http://www.ccp4.ac.uk ***
>
>
> Hi, All,
>
> I just got a crystal and checked it can diffract.However, the
> resolution is low (3.1 A) and the mosaicity is about 1.1.Right now
> the
> crystallization condition is quite simple, just use the AS with
> several acid pH. Is there anything I can do for the next step to
> improve the mosaicity and then hopefully improve the resolution?Is
> it
> related to the purity of my protein?When I set up trays, I noticed
> that the big crystals come from the impure sample.I only got tiny
> crystals from one of the pure sample and it grows really slow ( ~ 1
> week ).
> Thanks very much.
>
> Jenny
>
