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Would someone share their experience on making imidazole-malate buffer? Specifically, would be great to know the useful pH range as well as how to adjust pH within that range. Should the stock solutions of 2 M Imidazole and Malic Acid be adjusted to specific pH's prior to mixing in respective ratios? Thanks in advance and apologies for indirect relation of the question to CCP4. Vaheh Oganesyan, PhD Scientist II MedImmune, Inc. One MedImmune Way Gaithersburg, MD 20878 USA Phone: (301)398-5851 Facsimile: (301)398-9851 www.medimmune.com