Dear all:
We already got nice crystals of a drug-protein complex, however, MR
failed due to the huge copies (>12) of protein molecules per asu. Protein
itself is a small one, only ~70 aa.
Later on, we collected MAD data of copper (copper : protein ~ 1: 1),
Rsym of the data was around ~9%, the anomalous signals were weak, and only
good to ~6A, the data also failed to solve the phases.
Thus, basically, the Cu anomallous signal is very weak, and the
crystals are kind of sensitive to radiation, it is dying but not that fast.
I am wondering, Do we stand any chances to solve the phases by Cu MAD
or SAD?
Any suggestions or comments are highly appreciated?
Yi
