Regarding stochastic processes: to increase the chances of nucleation
one would like to have protein in the right nucleation zone for a
certain period of time.
If the drop becomes too small, the ratio evaporation surface/drop
volume changes. So, the nucleation zone might be reached within the
drop but either too fast ('racing through the phase diagram too
quickly') or in the smaller droplet turbulence caused by evaporation
might be more pronounced and interfere with nucleation.
At some stage I looked at this myself, although not for tiny volumes
(0.3, 0.5 and 0.8ul droplets). With smaller volume I saw more and
faster nucleation, but less crystal growth. There's got to be an
optimum in terms of drop volume when using vapour diffusion. Any
comments?
Carien
On 16 Jan 2008, at 13:17, Anastassis Perrakis wrote:
Oryxnano 50+50 nL
Demetres
Which, indirectly, brings up an interesting (but not relevant to the
Oryx) question.
Nucleation is a process that does have a stochastic aspect.
Thus, one could argue that compromising to 200-300 nl might be better
than either extremes of 50nl (too small volume and less chance for
nucleation) or 1000 nl (too much sample).
any comments ? (let the flames begin).
A.
PS1
another interesting issue that has has been hardly touched in these
emails is the real sample loss: left in wells and not easy to recover,
lost because of contamination with system liquid, etc ...
PS2
I see lots of people with new robots. please do have a look at the
www.BIOXHIT.org page and if you have a few minutes to assemble a table
we will be happy to add your specs to our pages. it can be a nice
resource and it has already enough things and already one response to
my last email ;-) To make life easier to potential contributors we can
provide an Excel sheet to fill up with your specs - just ask.
On Jan 16, 2008, at 12:46, Demetres D. Leonidas wrote:
David Briggs wrote:
I'll defend the honour of the phoenix... (again)
Bernhard Rupp 100+100 nl
Dave Briggs (and all users at Univ of Manchester, UK) 100+100nl
Others..
Only time we have ANY problems is when the nano dispensing tip gets
clogged. Often a good wash whilst still on the machine will clear
the blockage.
Dave
--
============================
David C. Briggs PhD
Father & Crystallographer
http://www.dbriggs.talktalk.net <http://www.dbriggs.talktalk.net>
AIM ID: dbassophile
============================
--
Demetres D. Leonidas, Ph.D.
Structural Biology & Chemistry Group
Institute of Organic and Pharmaceutical Chemistry
The National Hellenic Research Foundation
48, Vassileos Constantinou Avenue
Athens 116 35, Greece
==================================================
Tel. +30 210 7273841 (office)
+30 210 7273895 (lab) Fax. +30 210 7273831
E-mail: [EMAIL PROTECTED]
URL: http://athena.eie.gr
==================================================
The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company
Limited by Guarantee, Registered in England under Company No. 534147 with its
Registered Office at 123 Old Brompton Road, London SW7 3RP.
This e-mail message is confidential and for use by the addressee only. If the
message is received by anyone other than the addressee, please return the
message to the sender by replying to it and then delete the message from your
computer and network.
