Hi Joe,
We have a 32-head Honeybee robot which sets up a 96-well plate with a
single drop per well in ~6 minutes and 3 drops per well at ~9 minutes. A
96-head phoenix or hummingbird-like system is likely going to be faster,
but not by that much. Our robot has its own humidified cabinet and as
long as you keep evaporation under control I don't think set-up speed,
within reason, is that important.
Bart
JOE CRYSTAL wrote:
Hi,
Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots available? Besides cost per tray
and maintenance cost, another important feature we consider is the time
for setting up a 96-well tray. It is an important factor since we are
talking about sub-microliter drops.
Best,
Joe
On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy <[EMAIL PROTECTED]
<mailto:[EMAIL PROTECTED]>> wrote:
Al's Oil on the plates:
What a nightmare!!!!!!!
The oil creeps up the plate and over the sides. It dissolves adhesives.
It makes me say bad words in multiple languages.
Bigger drops + no oil = fewer bad words.
Lisa
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
[EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>
-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK
<mailto:CCP4BB@JISCMAIL.AC.UK>] On Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot
One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops. Two suggestions: (1) increase the
proportion of protein in the - technical term - teeny drop to say two
thirds and (2) cover the drops with oil eg Al's oils
(silicone/paraffin). You still get vapor diffusion though the oil ,
and you'd like to slow up equilibration. of course (2) slows up the
robotics a little, but both should be trivial to set up..
--
==============================================================================
Bart Hazes (Assistant Professor)
Dept. of Medical Microbiology & Immunology
University of Alberta
1-15 Medical Sciences Building
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Canada, T6G 2H7
phone: 1-780-492-0042
fax: 1-780-492-7521
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