Dear CCP4 experts, I'm working on a kind of cystein peptidase. It shows a little degradation after 2 days storing at 4 degree even I tried many kinds of protease inhibitors, or glycerol and DTT. So I decided to work fast (1 day) then set xtal. After one week I pick up some condition and check degradation by SDS-PAGE. It shows heavy degradation then I think it's impossible to obtain the crystal if I don't find out how to stop the degradation first.
Do you have any idea about my situation? Is it true that I need to stop the degradation and how to deal with it? Thank you for your advices, My best regards, TriNgo
