If your protein is autolyzing, you may need to crystallize it in the
presence of an inhibitor. In the case of trypsin, I have crystallized
in the presence of benzamidine, then removed the inhibitor by dialysis
after crystallization.
kmj
Ngo Duc Tri wrote:
Dear CCP4 experts,
I'm working on a kind of cystein peptidase. It shows a little
degradation after 2 days storing at 4 degree even I tried many kinds of
protease inhibitors, or glycerol and DTT.
So I decided to work fast (1 day) then set xtal. After one week I pick
up some condition and check degradation by SDS-PAGE. It shows heavy
degradation then I think it's impossible to obtain the crystal if I
don't find out how to stop the degradation first.
Do you have any idea about my situation? Is it true that I need to stop
the degradation and how to deal with it?
Thank you for your advices,
My best regards,
TriNgo
