I used glutaraldehyde vapor by placing crystal drop above a bridge with 15% glutaradehyde for various amount of time. It worked for me for many crystals, but not for the one that I am trying now. The crystal didn't diffract any more. I intended to make the crystal tougher for heavy metal soaking. I am just wondering what will happen if I use some other crosslinking chemicals.
Nian Huang Dept of Biochemistry UT Southwestern Medical Center Dallas, TX 75390 On Sun, Mar 2, 2008 at 12:28 AM, Nian Huang <[EMAIL PROTECTED]> wrote: > Dear all, > I know most people use glutaraldehyde as the crosslinking reagent for > their crystals. But there are a lot of other crosslinking chemicals > available both in protein chemistry and histology, such as simple > formaldehyde. Did anybody have experience with other chemicals and how > did they compared with glutaradehyde? I have a crystal very sensitive > to glutaradehyde but I really wants to try the crosslinking method. > Thanks, > > Nian Huang > Dept of Biochemistry > UT Southwestern Medical Center > Dallas, TX 75390 >
