I will try that. But I don't think people can tell the final concentration of glutaraldehyde in the drop by using its vapor. Maybe using direct soaking is what I should do. By the way, should I quench it by ammonium or not?
Nian Huang Dept of Biochemistry UT Southwestern Medical Center Dallas, TX 75390 On Sun, Mar 2, 2008 at 12:28 AM, Nian Huang <[EMAIL PROTECTED]> wrote: > Dear all, > I know most people use glutaraldehyde as the crosslinking reagent for > their crystals. But there are a lot of other crosslinking chemicals > available both in protein chemistry and histology, such as simple > formaldehyde. Did anybody have experience with other chemicals and how > did they compared with glutaradehyde? I have a crystal very sensitive > to glutaradehyde but I really wants to try the crosslinking method. > Thanks, > > Nian Huang > Dept of Biochemistry > UT Southwestern Medical Center > Dallas, TX 75390 >
