Hi, I have a question with B-factor and TLS refinement. My protein diffracts only to modest/low resolutions (3.4 A). I've been refining isotropic B-factors for main chain and side chain (not individual atoms) because I'm worried at this resolution I don't have enough observations to justify individual B-factor refinement.
First question: Is this a good assumption on my part, or should I try refining individual B-factors and look at how R/Rfree behaves? I just don't want to be over-refining my structure. Second question: Can I do TLS refinement at low resolution when B-factors are only refined for main chain and side chain? I would imagine that I need to be refining individual B-factors. Third question: Is it justified for me to be doing TLS refinement at these resolutions? Thanks Alexei