> ps. in passing, it seems like it would be a great idea to get together an > excel database of all false-positive results (e.g. phosphate salt crystals) > commonly found in the usual crystal screens. One could then search it to see > whether one's current crystallization conditions have be villified in the > past.
Good idea - I've set up a page on CCP4 wiki to record such occurrences: http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Conditions_prone_to_salt_cyrstallization In theory, none of the screens shall produce salt crystals unless a) there is something in your protein buffer or b) drops dried out. So report you protein buffer content and don't report cases of salt crystals in 3 years old trays. -- Edwin Pozharski, PhD, Assistant Professor University of Maryland, Baltimore ---------------------------------------------- When the Way is forgotten duty and justice appear; Then knowledge and wisdom are born along with hypocrisy. When harmonious relationships dissolve then respect and devotion arise; When a nation falls to chaos then loyalty and patriotism are born. ------------------------------ / Lao Tse /
