As an undergrad late in the last century, I used a micromanipulator,
quartz capillaries, and a device similar to a patch pipet (manually
operated via a screw) to do just this. It was just about the time that
nylon loops were coming into wide use, though, and I gladly abandoned he
capillary method in favor of loops. For microcrystals, I favor using
micromesh mounts from MiTeGen.
--
Kevin M. Jude
Berger Lab
California Institute for Quantitative Biosciences
University of California, Berkeley
On 3/26/09 12:43 PM, Jacob Keller wrote:
Dear Crystallographers,
Has anybody ever heard of mounting crystals in tiny crystal-sized
capillaries, such as are pulled by patch-pipet machines, or those used
in microfluidics? The material could be either glass or plastic, and one
could have some method of continuous positive or negative pressure,
perhaps through a hole in the crystal cap. Anyway, once safely inside
the tiny capilary, one could freeze it at leisure, without concern for
evaporation. It would really make harvesting easy--just vacuum up the
crystal, then plop in LiqN2/propane as per usual. I guess it could also
really be done with appropriate modification of a micro-manipulator.
Jacob
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu <mailto:j-kell...@northwestern.edu>
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