Dear Chris,
I recall from many years ago that we had a screening hit in high
isopropanol, which started boiling violently when we opened the well. We
"solved" the problem by using another precipitant but I would also be
interested in tricks how to handle high isopropanol.
Best regards,
Herman
________________________________
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On
Behalf Of Chris Meier
Sent: Friday, April 09, 2010 10:39 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] freezing crystals grown in isopropanol
condition
Dear all,
I have a protein which crystallizes in 25% isopropanol, at
pH4.5.
Does anyone have experience freezing crystals grown in such a
condition?
What cryoprotectants should I try?
Can isopropanol itself act as a cryoprotectant?
Any suggestions on how to deal with isopropanol evaporation
during mounting?
Many thanks and best wishes,
Chris
