English et al. Recombinant and in vitro expression systems for hydrogenases: new frontiers in basic and applied studies for biological and synthetic H2 production. Dalton Trans. (2009) (45) pp. 9970-8
I've never tried to express a hydrogenase, but I've read that it's very hard. Do you have all necessary assembly proteins and/or are you expressing in an organism that has a closely related hydrogenase? -Joel On Wed, May 5, 2010 at 8:14 AM, xaravich ivan <xaravich.i...@gmail.com>wrote: > I have found that if you give a cold shock ( 4 degrees for 30 mins-1 hr) > before low temperature induction it helps to keep proteins > soluble. > > Ivan > > > > > >
