Hello Kornelius, In 2 or 3 cases I successfully used a homology model for molecular replacement using a model generated from the PHYRE server http://www.sbg.bio.ic.ac.uk/~phyre/. I know it is not the same as ROSETTA but if there is some sequence homology you might be lucky...
Best regards Eike Am 25.08.10 10:13 schrieb "Kornelius Zeth" unter <kornelius.z...@tuebingen.mpg.de>: > Dear all, > > I was wondering if anybody has used the ROSETTA software to generate a MR > model that could subsequently being used successfully for a MR solution case. > > The sequence of the protein we work with is relatively small, ~ 85 residues. > Crystallization is not very reproducible. Resolution is 1.9 A. Crystals are > extremely rare. > > I would be grateful for any hints and will send a summary of all personally > sent comments to the list. > > Thank you and have a nice day > > Kornelius > > ---------------------------------------------- > Kornelius Zeth > Max Planck Institute for Developmental Biology > Dept. Protein Evolution > Spemannstr. 35 > 72076 Tuebingen, Germany > kornelius.z...@tuebingen.mpg.de > Tel -49 7071 601 323 > Fax -49 7071 601 349
