Hi Kornelius, A possible solution at this resolution would be to use Arcimboldo which localizes model fragments such as small helices with Phaser and then does density modification with SHELXE. See:
Nature Methods 6:651-3. "Crystallographic ab initio protein structure solution below atomic resolution" Peter. On Wed, Aug 25, 2010 at 10:13 AM, Kornelius Zeth < kornelius.z...@tuebingen.mpg.de> wrote: > Dear all, > > I was wondering if anybody has used the ROSETTA software to generate a MR > model that could subsequently being used successfully for a MR solution > case. > > The sequence of the protein we work with is relatively small, ~ 85 > residues. Crystallization is not very reproducible. Resolution is 1.9 A. > Crystals are extremely rare. > > I would be grateful for any hints and will send a summary of all personally > sent comments to the list. > > Thank you and have a nice day > > Kornelius > > ---------------------------------------------- > Kornelius Zeth > Max Planck Institute for Developmental Biology > Dept. Protein Evolution > Spemannstr. 35 > 72076 Tuebingen, Germany > kornelius.z...@tuebingen.mpg.de > Tel -49 7071 601 323 > Fax -49 7071 601 349 > -- Peter
