On 25/08/10 23:24, Garib Murshudov wrote:
However 1) coot uses 2mFo-DFc maps
Typically yes, but it uses whatever Refmac (or other programs) provide (of course)
2) you should be able to feed any map you want to coot
Yes.
so it is nice place for experimenting this kind of calculation
Hopefully.
3) You may try to relax gemetry
R/RC -> Refinement Weight. (I often change it to 6 (from 60) if I need to tighten things up.)
4) Usually if the model does not fit into electron density and programs do not want to correct it then there may be some fundamental problem in this part of the model
Agreed! On 25/08/10 23:13, Hailiang Zhang wrote:
Actually I tried coot real space refine zone, but the model seems not sliding into the best density map
Can you expand on that? On 25/08/10 23:33, Pavel Afonine wrote:
Compare slides #4 and #5: http://cci.lbl.gov/~afonine/rsr.pdf
Yes, slide 5 is what Coot uses. Paul.
