Michael, We have put together a review about use of folding modulators to improve expression in E. coli some time ago which might be useful to you (Kolaj et al Microb Cell Fact. 2009 Jan 27;8:9). Here is the link: http://www.ncbi.nlm.nih.gov/pubmed/19173718
<http://www.ncbi.nlm.nih.gov/pubmed/19173718>Regards On Fri, Apr 22, 2011 at 4:28 PM, Kothe, Michael <michael.ko...@genzyme.com>wrote: > Dear ccp4bb, > > > > I am curious to hear of examples where the expression of well-behaved > protein was achieved by the coexpression of chaperones in E. coli. I know > the appropriate strains and vectors exist, but I can’t remember hearing of a > successful case. I have heard anecdotally of several cases where it was > tried without success (including one attempt I made myself). I also heard of > concerns that the chaperones might copurify with the (now soluble) protein > of interest and are difficult to remove. > > > > Thanks, > > Michael > > >
