Hi,
I wanted to thank everyone for their responses to my inquiry. It's good to see that there have been positive examples, and also that one should not be discouraged from trying by initial negative results. Thanks again, and have a nice weekend, Michael ________________________________ From: Olga Kolaj [mailto:olga.ko...@gmail.com] Sent: Tuesday, April 26, 2011 5:17 AM To: Kothe, Michael Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] Using chaperones to boost expression in E. coli Michael, We have put together a review about use of folding modulators to improve expression in E. coli some time ago which might be useful to you (Kolaj et al Microb Cell Fact. 2009 Jan 27;8:9). Here is the link: http://www.ncbi.nlm.nih.gov/pubmed/19173718 <http://www.ncbi.nlm.nih.gov/pubmed/19173718> Regards On Fri, Apr 22, 2011 at 4:28 PM, Kothe, Michael <michael.ko...@genzyme.com> wrote: Dear ccp4bb, I am curious to hear of examples where the expression of well-behaved protein was achieved by the coexpression of chaperones in E. coli. I know the appropriate strains and vectors exist, but I can't remember hearing of a successful case. I have heard anecdotally of several cases where it was tried without success (including one attempt I made myself). I also heard of concerns that the chaperones might copurify with the (now soluble) protein of interest and are difficult to remove. Thanks, Michael
