On Fri, 2011-09-30 at 10:49 -0400, Patrick Loll wrote:
> Has anyone encountered a case in which a construct with the native
> sequence expressed poorly (or not at all?) in Rosetta(DE3), but the
> corresponding construct with a codon-optimized sequence expressed
> well? (The gene in question is from cerevesiae)
>
Wait, isn't Rosetta optimized for mammalian genes, not yeast (but maybe
codon bias in yeast matches that)?
A sideways suggestion would be to express in yeast. Extra bonus - it
smells like beer :)
--
"Hurry up before we all come back to our senses!"
Julian, King of Lemurs
