We are trying to determine Tm value of rather unstable proteins with a Tm in the mid 20 C range using DSF/thermofluor. Our Stratagene thermocycler has a low temp limit of 25 C (Peltier). I called the company and they said it's a 'hardware limit' that cannot be changed.
1) Has anyone been able to 'hotwire' the MX3000/3005 to go below this limit? 2) Are there other thermofluor machines that allow lower starting temperatures (say 4, 10 or 15 C)? Best regards, -Reggie
