Lots of systems go to 4C. Even a cheapo one like the Applied Biosystems StepOnePlus.
-Morten Groftehauge On 27 October 2011 03:17, Jürgen Bosch <jubo...@jhsph.edu> wrote: > CD spec with Pelletier is an option too > > Jürgen > > ...................... > Jürgen Bosch > Johns Hopkins Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns Hopkins Malaria Research Institute > 615 North Wolfe Street, W8708 > Baltimore, MD 21205 > Phone: +1-410-614-4742 > Lab: +1-410-614-4894 > Fax: +1-410-955-3655 > http://web.mac.com/bosch_lab/ > > On Oct 26, 2011, at 19:08, Reginald McNulty <rmcnu...@uci.edu> wrote: > > We are trying to determine Tm value of rather unstable proteins with a Tm > in > the mid 20 C range using DSF/thermofluor. Our Stratagene thermocycler has > a > low temp limit of 25 C (Peltier). I called the company and they said it's > a > 'hardware limit' that cannot be changed. > > 1) Has anyone been able to 'hotwire' the MX3000/3005 to go below this > limit? > > 2) Are there other thermofluor machines that allow lower starting > temperatures (say 4, 10 or 15 C)? > > Best regards, > -Reggie > > > >
