Thanks for all the replies -- there are no suggestions in the
literature or in crystallized or predicted domain structures that this
protein binds a cofactor, and, although I did purify it in insect
cells, PAGE gels and activity assays support the assertion that it's
not ferritin. Nobody has seen any metal ions bound, either, but there
are a few domains that haven't been crystallized, so maybe. Again,
thanks for all the possibilities; will keep them in mind.
Caitlyn
On Nov 5, 2011, at 5:02 PM, Caitlyn Claire Yeykal wrote:
Hi -- has anyone had crystals that are colored in regular
(unpolarized) light? Mine are yellow, and I'm not aware of anything
in the buffer conditions that might cause this. I read online that
glutaraldehyde can turn protein crystals a golden color, but as far
as I know there isn't any of that in the well. Purified in HBS pH
7.2; crystallized in LiCl/PEG4K/Tris pH 8. Any explanations?
Thanks!
Caitlyn
____________________________________
Caitlyn C. Yeykal
Mrksich Group/Adams Group
Dept. of Biochemistry, University of Chicago
929 E. 57th St., Rm 547B
Chicago, IL 60615
cait...@uchicago.edu
