In another thread, you indicated that there were no identifiable cofactor binding sites in your protein, so we are down to less common situations. Some proteins are spontaneously decorated with pyridoxal on surface lysine residues. In some cases, this has absolutely nothing to do with the enzymatic activity of the protein.
On Nov 5, 2011, at 5:02 PM, Caitlyn Claire Yeykal wrote: > Hi -- has anyone had crystals that are colored in regular (unpolarized) > light? Mine are yellow, and I'm not aware of anything in the buffer > conditions that might cause this. I read online that glutaraldehyde can turn > protein crystals a golden color, but as far as I know there isn't any of that > in the well. Purified in HBS pH 7.2; crystallized in LiCl/PEG4K/Tris pH 8. > Any explanations? > > Thanks! > Caitlyn > > ____________________________________ > Caitlyn C. Yeykal > Mrksich Group/Adams Group > Dept. of Biochemistry, University of Chicago > 929 E. 57th St., Rm 547B > Chicago, IL 60615 > cait...@uchicago.edu
