Ditto to Poul's advice. I've had many many many cases where crystals diffract poorly (or not at all) on home sources only to show excellent diffraction at a synchrotron. (Whether or not a home source is properly calibrated is probably the biggest issue, but that's for another discussion).
On Jan 26, 2012, at 8:33 AM, Theresa H. Hsu wrote: > Dear crystallographers > > I have a protein of 90 kDa forming dimers. Crystals formed with microbatch > and vapor diffusion method in 24 hours but no diffraction at home source. > Dissolved crystals was confirmed to be the protein with mass spec. > > Any suggestions to improve diffraction would be welcome. > > Thanking you in advance. > > Theresa --------------------------------------------- Francis E. Reyes M.Sc. 215 UCB University of Colorado at Boulder
