Maybe, you can adjust the ion strength of your condition.



On Thu, Jan 26, 2012 at 8:32 AM, Kevin Jin <kevin...@gmail.com> wrote:
> For crystallization:
>
> Your xtal may come out a little bit fast. If the condition contain
> alcohol,  such as IPA, you may have to modify it.
>
> If you let people know the condition, it may be more helpful.
>
> Also, please check the purity of your protein.
>
> Kevin
>
> On Thu, Jan 26, 2012 at 7:33 AM, Theresa H. Hsu <theresah...@live.com> wrote:
>> Dear crystallographers
>>
>> I have a protein of 90 kDa forming dimers. Crystals formed with microbatch 
>> and vapor diffusion method in 24 hours but no diffraction at home source. 
>> Dissolved crystals was confirmed to be the protein with mass spec.
>>
>> Any suggestions to improve diffraction would be welcome.
>>
>> Thanking you in advance.
>>
>> Theresa

Reply via email to