1. Try room temperature mounts (as suggested by others) 2. Expose the hell out of the crystal (5 min) on home source or go synchrotron 3. Run your protein through another column (ion exchange) even if it looks pure 4. Try an additive screen 5. Try limited proteolysis or methylation
6. If none of theat works, clone the same protein from another organism/strain/variant, ad try again
