Another good lesson here:
2. The SAXS solution structure of RF1 differs from its crystal structure and is similar to its ribosome bound cryo-EM structure. Vestergaard B, Sanyal S, Roessle M, Mora L, Buckingham RH, Kastrup JS, Gajhede M, Svergun DI, Ehrenberg M. Mol Cell. 2005 Dec 22;20(6):929-38. On 11/02/2012, at 18.18, Joel Sussman wrote: > 2012_02_11 > Dear All, > Two really striking examples of "Intrinsically Flexible Proteins" are: > > (1) Adenylate kinase: Vonrhein, Schlauderer & Schulz (1995) Structure 3, 483 > “Movie of the structural changes during a catalytic cycle of nucleoside > monophosphate kinases” > http://portal.uni-freiburg.de/structbio/structuregallery/ak_folder/mpeg > in particular look at: > "video as MPEG white background, closing & opening enzyme (707kb)" > Each "black dot" [upper left, in the morph] indicates an observed crystal > structure. > > (2) Lac repressor: see Proteopedia page on lac repressor, > morphing from the structure bound to its cognate DNA, to that of the > structure bound to its the non-cognate DNA, > at: http://proteopedia.org/w/Lac_repressor > > best regards, > Joel > > > On 10 Feb 2012, at 22:51, Jacob Keller wrote: > >> Interesting to juxtapose these two responses: >> >> James Stroud: >>> How could they not be snapshots of conformations adopted in solution? >> >> David Schuller: >>> How could that possibly be the case when any structure is an average of all >>> the unit cells of the crystal over the timespan of the diffraction >>> experiment? >> >> JPK >> >> >> >> ******************************************* >> Jacob Pearson Keller >> Northwestern University >> Medical Scientist Training Program >> email: j-kell...@northwestern.edu >> ******************************************* >
