Any thoughts on determining flexible regions, trying mutations, truncations, different sequences from difference species? It is essentially the protein that crystallizes, so I tend to think of making changes to the protein itself.
From: ruby singh Sent: Friday, May 04, 2012 3:09 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Dear all, Im a PhD student who started working on Protein crystallization. Its been years im trying to get any crystal of that protein. I have tried all crystallization screens available. but no results. My protein is highy thermostable(till 80C) and pH stable(1 to 13)...plz if anyone is having any idea or trick let me knw.
