That is probably because you pH Tris with HCl rather than HEPES with NaOH. The Ksp for Zn(OH)2 is 3x10^17 so the excess hydroxides are probably what are killing your solution.
Cheers, Katherine On Fri, May 11, 2012 at 11:43 AM, Rajesh Kumar <ccp4...@hotmail.com> wrote: > If there is no cure , then fine. > pH may not be the answer as it doesn't Happen with TRIS buffer pH 7.6. > Thanks to every one > Rajesh > > > ------------------------------ > Date: Fri, 11 May 2012 12:35:45 -0400 > From: dj...@cornell.edu > > Subject: Re: [ccp4bb] zinc with HEPES > To: CCP4BB@JISCMAIL.AC.UK > > > > There is no cure for HEPES. > > > > > -- > ======================================================================= > All Things Serve the Beam > ======================================================================= > David J. Schuller > modern man in a post-modern world > MacCHESS, Cornell University > schul...@cornell.edu > >
