The rationale was to see if Zn could make differences in crystal morphology.
This is because the protein has CxxC and CxxH similar to a zinc finger
motif.All my efforts, additive screening, MMS, streaking, micro batch, hanging
drop, changing drop ratio, drop shape, did not help me to either increase
thickness or change the shape of very very thin needle crystals.
Yes, I will try very less, 50uM.Thanks for helping me to understand.Rajesh
> Date: Fri, 11 May 2012 12:53:53 -0400
> Subject: Re: [ccp4bb] zinc with HEPES
> From: liehy...@gmail.com
> To: ccp4...@hotmail.com
>
> Rajesh,
> 10mM zinc seems a bit too high. I normally used it at <50uM conc.
> ray
>
> On Fri, May 11, 2012 at 12:26 PM, Rajesh Kumar <ccp4...@hotmail.com> wrote:
> > Dear All,
> >
> > This question sounds simple but I dont know the answer.
> > I was preparing a 24 well crystal screen. When I try to use 10 mM ZnSO4
> > with HEPES (pH 7.6) buffer it precipitates. I tried both ZnCl2 and Zn
> > acetate the effect is same.
> > I dont know why this Zn in not compatible with HEPES.
> > Could you please tell me why is this?
> > I appreciate your help.
> >
> > Thanks
> > Rajesh
