I and Victor Lamzin solved our first protein structure (3A resolution) in 80-s using pure MIR and a home made (Russian) diffractometer...
Alex On Jun 6, 2012, at 1:42 PM, Boaz Shaanan wrote: > So if get the gist of the thread right, am I correct in assuming that the > last protein structures to be solved strictly by MIR are > haemoglobin/myoglobin, lysozyme and chymotrypsin and perhaps one or two more > in the late sixties? In which case the answer to the original question about > MIR being obsolete, is "yes it is since a long time"? > > Boaz > > > Boaz Shaanan, Ph.D. > Dept. of Life Sciences > Ben-Gurion University of the Negev > Beer-Sheva 84105 > Israel > > E-mail: bshaa...@bgu.ac.il > Phone: 972-8-647-2220 Skype: boaz.shaanan > Fax: 972-8-647-2992 or 972-8-646-1710 > > > > > > ________________________________________ > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Phil Evans > [p...@mrc-lmb.cam.ac.uk] > Sent: Wednesday, June 06, 2012 6:04 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Fun Question - Is multiple isomorphous replacement an > obsolete technique? > > No they were not useless! I used them > > (probably better now with cryo data though) > > Phil > > On 6 Jun 2012, at 16:02, Dyda wrote: > >>> I suspect that pure MIR (without anomalous) was always a fiction. I doubt >>> that anyone has ever used it. Heavy atoms always give >>> an anomalous signal >> >>> Phil >> >> I suspect that there was a time when the anomalous signal in data sets was >> fictional. >> Before the invent of flash freezing, systematic errors due to decay and the >> need >> of scaling together many derivative data sets collected on multiple crystals >> could render >> weak anomalous signal useless. Therefore MIR was needed. Also, current >> hardware/software >> produces much better reduced data, so weak signals can become useful. >> >> Fred >> >> ?[32m******************************************************************************* >> Fred Dyda, Ph.D. Phone:301-402-4496 >> Laboratory of Molecular Biology Fax: 301-496-0201 >> DHHS/NIH/NIDDK e-mail:fred.d...@nih.gov >> Bldg. 5. Room 303 >> Bethesda, MD 20892-0560 URGENT message e-mail: 2022476...@mms.att.net >> Google maps coords: 39.000597, -77.102102 >> http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/DydaFred >> *******************************************************************************?[m
