Hi Samuel, I've has good success going from sphereulites to crystals using an additive screen (the 96 condition Hampton one is good) with the conditions giving the spherulites. Just watch for salt crystals as you'll be adding some compounds that might cause your Ca ions to form insoluble CaSO4.
http://hamptonresearch.com/product_detail.aspx?cid=1&sid=36&pid=27 Cheers, Rhys ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Samuel Johnson [samueljohnson...@yahoo.in] Sent: 25 August 2012 02:11 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Improving microcrystals Hi everyone, I have been working on a protein for the past year. After a number of trials at crystallizing the protein i have identified conditions for getting spherulites/micro-crystaline material under micro batch method. I have confirmed that the crystalline material is protein, by using Izit-dye test. The condition is 50mM CaCl2, Mes pH 6.5 and 40% PEG 400. I will be happy to get suggestions on improving conditions to obtain single crystals. I have already tried varying a number of parameters like salt, precipitant concentration and buffer pH but that didn't help. Thanks.
