I've had some success in the past following Terese Bergfors' advice: http://xray.bmc.uu.se/terese/tutorial3.html
However, the last time I dealt with spherulites I just tuned the pH and it worked like a charm. Good luck, Jon 2012/8/25 RHYS GRINTER <r.grinte...@research.gla.ac.uk> > Hi Samuel, > > I've has good success going from sphereulites to crystals using an > additive screen (the 96 condition Hampton one is good) with the conditions > giving the spherulites. Just watch for salt crystals as you'll be adding > some compounds that might cause your Ca ions to form insoluble CaSO4. > > http://hamptonresearch.com/product_detail.aspx?cid=1&sid=36&pid=27 > > Cheers, > > Rhys > ________________________________________ > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Samuel > Johnson [samueljohnson...@yahoo.in] > Sent: 25 August 2012 02:11 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Improving microcrystals > > Hi everyone, > > I have been working on a protein for the past year. > After a number of trials at crystallizing the protein i have identified > conditions for getting spherulites/micro-crystaline material under micro > batch method. I have confirmed that the crystalline material is protein, by > using Izit-dye test. The condition is 50mM CaCl2, Mes pH 6.5 and 40% PEG > 400. I will be happy to get suggestions on improving conditions to obtain > single crystals. I have already tried varying a number of parameters like > salt, precipitant concentration and buffer pH but that didn't help. > > Thanks. > -- Jon Agirre, PhD Unit of Biophysics (CSIC-UPV/EHU) http://www.ehu.es/jon.agirre http://sourceforge.net/projects/projectrecon/ +34656756888
