Acoot You may be interested in some data-mining that we did a few years ago
http://www.douglas.co.uk/PDB_data.htm#Acid-base We didn't see any obvious correlations On 28 October 2012 03:00, Acoot Brett <acootbr...@yahoo.com> wrote: > Dear All, > > A protein crystal can be got at pH 5 or 8, or a pH with much extreme > value. What will be the relatively extreme pH value to get the crystal on > the protein structure solved based on the crystal got? I mean usually we > regard the physiological pH as 7. If a crystal was got at pH 5, the > structure solved may be different from the protein structure at pH 7. But > it seems there is rarely analysis on the discrepancy of the protein > structures when publishing 3-D structure with the protein crystal got at > relatively extreme pH. > > I am looking forward to getting your comment on it. > > Cheers, > > Acoot -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
