Hi, These sorts of questions are always difficult, particularly in the absence of any information about the protein or the contents of the mother liquor. If the carbonyl you are talking about is the little magenta dot visible through the hole in your blob, this could be a metal atom with some long chelating molecule around the equator. In the extreme it could be some sort of porphyrin, although the density would be very poor if it was.
Dale Tronrud On 11/28/2012 7:48 AM, Read, Jon wrote:
Anyone see anything like this before? The data is 1.7Angstrom data with good statistics. The picture shows the solid FoFc density contoured at 3 Sigma in light brown and -3 Sigma in purple. The density is odd as it appears to be bound to a peptide carbonyl with no other obvious interactions with the protein. There is a characteristic tail at one end. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 2 Kingdom Street, London, W2 6BD. *Confidentiality Notice: *This message is private and may contain confidential, proprietary and legally privileged information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorised use or disclosure of the contents of this message is not permitted and may be unlawful. *Disclaimer:* Email messages may be subject to delays, interception, non-delivery and unauthorised alterations. Therefore, information expressed in this message is not given or endorsed by AstraZeneca UK Limited unless otherwise notified by an authorised representative independent of this message. No contractual relationship is created by this message by any person unless specifically indicated by agreement in writing other than email. *Monitoring: *AstraZeneca UK Limited may monitor email traffic data and content for the purposes of the prevention and detection of crime, ensuring the security of our computer systems and checking compliance with our Code of Conduct and policies.
