Feng Lei,
I would personally recommend slowing down the process of nucleation either by
lowering the temperature of crystallization or by utilizing Al's oil. These are
two powerful ways to slow the overall kinetics of crystallization. Here is a
relevant reference for you:
http://scripts.iucr.org/cgi-bin/paper?he0181
Sincerely, lorenzo
Lorenzo Ihsan Finci, Ph.D.Postdoctoral Scientist, Wang LaboratoryHarvard
Medical SchoolDana-Farber Cancer InstituteBoston, MA Peking UniversityCollege
of Life SciencesBeijing, China
Date: Mon, 25 Feb 2013 11:02:03 -0500
From: spartanfeng...@hotmail.com
Subject: [ccp4bb] How to slow down crystallization? Need hep!
To: CCP4BB@JISCMAIL.AC.UK
Hello everyone,
I need your suggestion for slowing down crystallization for my protein
my protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9), but it
crystallize too fast. In 1 hr I can see tons of tiny needles.
Can anyone give me some suggestion on how to slow down the process? I used
lower conc. of potein, lower conc. of PEG ( 10%), it helped a little bit,
giving me small rod crystal. but no improvement after that.
Thank you very much for your suggestions
