Le 25/02/13 17:02, lei feng a écrit :
Hello everyone,
I need your suggestion for slowing down crystallization for my protein
my protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9),
but it crystallize too fast. In 1 hr I can see tons of tiny needles.
Can anyone give me some suggestion on how to slow down the process? I
used lower conc. of potein, lower conc. of PEG ( 10%), it helped a
little bit, giving me small rod crystal. but no improvement after that.
Thank you very much for your suggestions
you can run crystallization at 4°C, or add agarose gel to your
crystallization drop.
--
Philippe Leone
AFMB-UMR7257
Team 'Structural Immunology'
Case 932, 163 Avenue de Luminy, 13288 Marseille cedex 9, France
Tel: +33 491 82 55 81
Fax: +33 491 26 67 20
e-mail: philippe.le...@afmb.univ-mrs.fr
