On 06/20/2013 06:20 AM, Eleanor Dodson wrote:
But you say you took the Balbes model into phaser? and I think Balbes
automatically runs cycles of refinement so any comment on R factors
may not mean much.
I have seen a model coming out of Balbes pipeline hitting extremely high
marks when fed into Phaser while being complete nonsense (it's a 150kDa
multi-domain protein and resulting domain arrangement made absolutely no
sense). Refinement was stuck with high R-values and I sadly gave up on
it for now. I suspected that refmac step included in the pipeline
artificially shifts the model so that it conforms to Patterson map
better, which results in high score in Phaser. However, when I tried to
reproduce this phenomenon by force-refining some random 12kDa protein
against lysozyme data and then feeding the result into Phaser, it did
not produce any high scores.
Cheers,
Ed.
--
Oh, suddenly throwing a giraffe into a volcano to make water is crazy?
Julian, King of Lemurs
